GPCR Structures: delta opioid and nociceptin receptors
There will come a point in time when a new GPCR structure doesn't 'make the grade' for Nature or Science, but hopefully that time is still a considerable way off. There are two new GPCR structures released recently - the nociceptin receptor 4ea3 and the delta-opioid receptor 4ej4, bringing it to a grand total of 15 sequence distinct rhodopsin-like GPCR structures in the public domain.
Both new ones published alongside two other previously released PDB entries in last weeks Nature.
Here is an alignment - thanks to the commenter on a previous post in this series that spotted my schoolboy error in a previous version.
- 3uon - human muscarinic M2 receptor
- 4daj - rat muscarinic M3 receptor
- 3rze - human histamine H1 receptor
- 2rh1 - human beta-2 adrenergic receptor
- 2vt4 - turkey beta-1 adrenergic receptor
- 3pbl - human dopamine D3 receptor
- 2ydv - human adenosine A2a receptor
- 3v2w - human sphingosine-1-phosphate receptor
- 4djh - human kappa opioid receptor
- 4dkl - mouse mu opioid receptor
- 4ej4 - mouse delta opioid receptor
- 4ea3 - human nociceptin receptor
- 3odu - human CCR4 receptor
- 2i35 - bovine rhodopsin
- 2z73 - squid rhodopsin
10 20 30 40 50 3uon ( 20 ) tfevvfivl 4dajA ( 64 ) iwqvvfiaf 3rze ( 28 ) mplvv 2rh1 ( 29 ) devwvvgmgi 2vt4A ( 40 ) weagmsl 3pblA ( 32 ) yal 2ydv ( 3 ) imgssvYit 3v2w ( 17 ) sdyvnydIIvrHYnyTgklnisa ltsv 4djhA ( 55 ) spaipviita 4dkl ( 65 ) mvtaitima 4ej4 ( 41 ) rsasslalaiaita 4ea3A ( 47 ) plglkvtIvg 3oduA ( 27 ) pçfre-------------------------enanfnkiflpt 1u19A ( 1 ) mnGtegpnfyVPfsnktgvVrsPFeapQyyLaepwqFsmlAa 2z73A ( 9 ) etwwyNpsIvVhpHWref--------------dqvpdavYyslGi aaaaaa 60 70 80 90 100 3uon ( 29 ) vagslSlvTiigNilVmvSIkvnrhLqtvnnyflfSLAcADliiGvfSMn 4dajA ( 73 ) ltgflAlvTiigNilVivAFkvnkqLktvnnyFllSLAcADliIGviSMn 3rze ( 33 ) vlsticlvTvglNllVlyAvrserkLhtvGnlYIvsLSvADliVGavVMp 2rh1 ( 39 ) vmslivlaIvfgNvlVitAIakferLqtvtnyFItsLAcADlvMGlaVVp 2vt4A ( 47 ) lmalVvllIvagNvlViaAigstqrLqtltnlFItsLAcADlvvGllVVp 3pblA ( 35 ) sYcalilaIvfgNglVcmAVlkeraLqtttnyLVvsLAvADllvAtlVMp 2ydv ( 12 ) vElaiavlAilgNvlVcwAvwlnsnLqnvtnyFVvsAAaADilVGvlAIp 3v2w ( 51 ) vfiliCcfIileNifvlltiwktkkFhrpMYyFIgnLAlSDllaGvaYta 4djhA ( 65 ) vysvvfvvGlvgNslVmfVIirytkmktaTniYIfNLAlADalVTtTMpf 4dkl ( 74 ) lYsiVcvvGlfgNflvmyvIvrytkMktAtniYIfNLAlADalATsTLpf 4ej4 ( 55 ) lYsavcavGllgNvlvmfgIvrytkLktATniYIfNLAlADalATstLpf 4ea3A ( 57 ) lYlavcvgGllgNclvmyVIlrhtkmktatNiYIfNLAlADtlVLlTLpf 3oduA ( 44 ) iYsiIfltGivgNglvilvMgyqkklrsmtdkYRlhLSvADllFVitLpf 1u19A ( 43 ) yMflLimlGfpiNflTlyVTvqHkkLrtplNyILlnLAvADlfMVfg-GF 2z73A ( 40 ) fIgiCgiiGcggNgiViyLFtktksLqtpanmFiinLAfSDftFSlvNGf aaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaaaa aaa 110 120 130 140 150 3uon ( 79 ) lytlytvi-gyWplgpvvÇdlWlalDYvVSNAsVmNLliiSfdryfcvtk 4dajA ( 123 ) lFttyiim-nrWalgnlaÇdlwLSiDYvASNAsVmNLlvISfDryfsitr 3rze ( 83 ) mnilyllm-skwsLgrplÇlfWLSmDYVASTASIfSVfiLCiDryrsvqq 2rh1 ( 89 ) fgaahilm-kmWtfgnfwçefWTSiDVlCVTASIeTLcvIAvdryfAIts 2vt4A ( 97 ) fgatlvvr-gtWlwgsflçelWTSlDVlCVTAsIeTLcvIAiDrylaits 3pblA ( 85 ) wvvylevtggvWnfsricÇdvFVTlDVmMcTAsIwNLCaISidRytAVvm 2ydv ( 62 ) faiaIst---GfçaaçhgÇLfiACfVLVLTASSIfSLlaIAiDryiairi 3v2w ( 101 ) Nlllsga--tTykLtPaqWFlREGsMFvALSASVfSLlaIAieryitmlk 4djhA ( 115 ) qstvylmn--sWpfgdvlÇkiVlsiDyyNMfTSIfTLtmMSvdRyiaVch 4dkl ( 124 ) qsvnylmg--tWpfgnilÇkiviSidYyNMFTSIfTLctMSvdRyiAVCh 4ej4 ( 105 ) qsakylme--tWpfgellÇkaVlSidYyNMFTSIfTLtmMSvDRyiavch 4ea3A ( 107 ) QGtdillg--fWpfgnalÇktVIaiDyyNMFTSTfTLtaMSvdryvaich 3oduA ( 94 ) WavDAva---nWyfgnflÇkaVHviYTVNlYSSVwILAfISlDRylAiVh 1u19A ( 92 ) tTTlyTSlhGyFvfgptGÇnlEGffATLGGEIaLWSLvvLaieRyvvVck 2z73A ( 90 ) plMtiSCflkkWifgfaaÇkvYGfiGGiFGFMsIMTMAMiSiDrynViGr aaaaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 160 170 180 190 200 3uon ( 128 ) pltypvk---rttkmAgmmiaaAwvlSfilwapaIlfwqfivg------- 4dajA ( 172 ) pltyrak---rttkrAgvmiglAwviSfvlWApaIlfwqyfvg------- 3rze ( 132 ) plrylky---rtktrAsatilgawflSfl-WvipIlgwnh 2rh1 ( 138 ) pfkyqSl---ltknkArviilmvwivSgltSflpIqmhwyr-----athq 2vt4A ( 146 ) pfryqsl---mtrarAkviictvwaiSalvSflpImmhwWr-----dedp 3pblA ( 135 ) pvhyqhgtgqsscrrValmitavwvlAfaVSc-pLlfgfNtTg------- 2ydv ( 109 ) plryngl---vtgtrAkgiiaicwvlSfaIGltPmlgwnnÇgqp--kegk 3v2w ( 156 ) nnfrlfllisacwviSlilGglPimgwn----------- 4djhA ( 163 ) pvkaldf---rtplkAkiinicIwllSssvGisAivlGGtkvred----- 4dkl ( 172 ) pvkaldf---rtprnAkivnvcNwilSsaiGlpVmfmAttkyrqg----- 4ej4 ( 153 ) pvkaldf---rtpakAklinicIwvlAsgvGvpimvmAvtqprdg----- 4ea3A ( 155 ) p tsskAqavnvaIwalAsvvGvpvaimGsAqvede----- 3oduA ( 141 ) atn---sqrprkllAekvVyvgVwipAlllT-ipDfif--Anvsead--- 1u19A ( 142 ) pmsn----frfgenhaimgvafTwvmAlaCAapPlvgwSrYIPE------ 2z73A ( 140 ) pmaas---kkMshrrAfimiifVwlwSvlwAigPifgwGaYtLE------ aaaaaaaaaaaaaaaaaaaa aaa 210 220 230 240 250 3uon ( 168 ) ----vrtVedgeÇyIqff------snaavtfgtAiaaFylpviiMtvlyw 4dajA ( 212 ) ----krtVppgeÇfIqfl------septitfgtAiaaFymPvtiMtilyw 3rze ( 175 ) rredkÇeTdfy------dvtwfkvmtaiinFylPtllMlwfya 2rh1 ( 180 ) eAinÇyae-etçÇdff--------TnqayaiasSivSFyvplviMvfvYs 2vt4A ( 188 ) qAlkçyqd-pgçÇdfv--------TnrayaiasSiiSFyipLliMifval 3pblA ( 177 ) --------dptvÇsIs---------npdFViySSvvSFylPfgvTvlvya 2ydv ( 154 ) ahsqgÇgegqvAÇlFedVV-----pmnYMVyfNffaCVlvPlllMlgvyl 3v2w ( 184 ) ----ÇisalssÇSTVLP-------LYhkhYIlfCTtvFtllllsIvilYc 4djhA ( 205 ) -------vdvieÇslqFpdddyswwdlfmkicVfifAfviPvliIivcyt 4dkl ( 214 ) ---------sidçtltfsh-ptwywenllKicVfifAfimPvliItvcyg 4ej4 ( 195 ) ---------avvÇmlqfps-pswywdtvtkicvflfAfvvPiliitvcyg 4ea3A ( 197 ) ---------eieÇlveipt-pqdywgpvfaiciflfSFivPvlvIsvcys 3oduA ( 182 ) --------dryiÇdrfyp---ndlwvvvfqfqhimvglilPgivIlsCyc 1u19A ( 182 ) -------GMQCSÇGIDYYTpheetnNesFViyMfvvHfiiPlivIffcyg 2z73A ( 181 ) -------GVLCNÇSFdYIsr--dsttrsNIlcMFilGffgPiliiffCyf aaaaaaaaaa aaaaaaaaaaaa 260 270 280 290 300 3uon ( 208 ) hisrasksri pppsrekkvtrtilaIllaFi 4dajA ( 252 ) rIyketek like aqTlsaIllaFi 3rze ( 212 ) kIykaVrqhc lhmnrerkaakQLgfIMaaFi 2rh1 ( 221 ) rVfqeakrql kfclkeHkaLktlgiIMgtFt 2vt4A ( 229 ) rvyreakeq irehkalktlgiImgvFt 3pblA ( 210 ) rIyvvlkqrrrk-----------------gvplrekkatqMVaiVlgaFi 2ydv ( 199 ) rIflaarrqlkqmesq stlqkevhaakSLaiIvglFa 3v2w ( 223 ) riyslvrtr asrssenvaLlkTViiVLsvFi 4djhA ( 248 ) lMilrlksvrllsg rekdrnlrritrLVlvVVavFv 4dkl ( 254 ) lmilrlksvr ekdrnlrritrMVlvVvavFi 4ej4 ( 235 ) lMllrlrsvr ekdrslrriTrMVlvVvgaFv 4ea3A ( 237 ) lMirrlrgvrlls-------------gsrekdrnlrritrLVlvVvavFv 3oduA ( 221 ) iIisklshs kghqkrkalktTviLilaFf 1u19A ( 225 ) qLvftvkeaaaq------------qqesattqkaekevTrMviiMviaFl 2z73A ( 222 ) nIvmsvsnhekemaamakrlnakelrkaqaganaemrlAkIsivIVsqFl aaaaa aaaaaaaaaaaaaaaaa 310 320 330 340 350 3uon ( 398 ) itWapYNvmVlintfçap--------ç--ipntvwtiGywlCYinstiNp 4dajA ( 501 ) itWtpyNimVlvntfçds--------ç--ipktywnlgywlCYiNStvNP 3rze ( 426 ) lCWipYFiffmviafçkn--------ç--cnehlhmftiWlGYiNStlNP 2rh1 ( 284 ) lcWlpFFiVNivhviqdn----------lirkevyillNwiGYvNSgfNp 2vt4A ( 301 ) lCWlpFFlvnivnvfnrd----------lvpdwlfvafnwlGYAnSAmnp 3pblA ( 340 ) vCWlpFFltHvlnthçqt--------ç-hvspelysattwlGYvNsalNP 2ydv ( 244 ) lCWlpLHiiNcftffçpd--------çshaplwlMylAivlSHtNSvvNP 3v2w ( 267 ) acwapLFiLLllDvgçkvk------tç--diLfrAeyfLvlAvlNSgtNP 4djhA ( 285 ) vcWtpIHifilvealgs aalssyyfcIalGytNSslNP 4dkl ( 291 ) vcWtpIHiyViikaliti-------pettfqtvswhfcialGYtNSclNp 4ej4 ( 272 ) vCWapIHifVivwtlvdi------nrrdplvvaalhlcialGYaNSslNp 4ea3A ( 274 ) gcWtpVQvfvlaqglgvq-------pssetavailrfctAlGYvNSclNp 3oduA ( 250 ) acWlpyyigisidsfilleiikqgçefentvhkwisitEAlAFfHCclNp 1u19A ( 263 ) iCWlpYAgvAfyIfthqgsd---------fgpifMTipAFfAKtSAvyNP 2z73A ( 272 ) lSWspYAvvAllAQfgplew---------VtpyaAQlpVMfAKaSaihNP aaaaaaaaaaaaaaa aaaaaaaaaaaaa aaa 360 370 380 390 400 3uon ( 438 ) acYalcnatFkktfkhllm 4dajA ( 541 ) vcYalcnktFrttfkt 3rze ( 466 ) liYplCnenFkktfkrilhi 2rh1 ( 324 ) liYc-rspdfriAfqellcl 2vt4A ( 341 ) iiYc-rspdfrkAfkrlla 3pblA ( 381 ) viYttfnieFrkAflkilsc 2ydv ( 286 ) fiyAyrireFrqTFrkiirshvlrqqepfkaa 3v2w ( 309 ) iiytltNkemrrafiri 4djhA ( 328 ) ilYafldenFkrcfrdfcfp 4dkl ( 334 ) vlYafldenFkrCfrefci 4ej4 ( 316 ) vlYaflDenfkrc 4ea3A ( 317 ) ilYafldenFkacfr 3oduA ( 300 ) ilyaflgakfktsaqhalts 1u19A ( 304 ) viYimmnkqFrnCmvttlccgknplgddeasttVsktetsqvapa 2z73A ( 313 ) miYsvsHpkFreAIsqtfpwvLtccqfddketeddkdaeteipage aaaaa aaaaaaaaaa 410
%T Crystal structure of the µ-opioid receptor bound to a morphinan antagonist %A A. Manglik %A A.C. Kruse %A T.S. Kobilka %A F.S. Thian %A J.M. Mathiesen %A R.K. Sunahara %A L. Pardo %A W.I. Weis %A B.K. Kobilka %A S. Granier %J Nature %V 485 %P 321–326 %O doi:10.1038/nature10954 %D 2012 %T Structure of the human κ-opioid receptor in complex with JDTic %A H. Wu %A D. Wacker %A M. Mileni %A V. Katritch %A G.W. Han %A E. Vardy %A W. Liu %A A.A. Thompson %A X.-P. Huang %A F.I. Carroll %A S.W. Mascarella %A R.B. Westkaemper %A P.D. Mosier %A B.L. Roth %A V. Cherezov %A R.C. Stevens %J Nature %V 485 %P 327–332 %O doi:10.1038/nature10939 %D 2012 %T Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic %A A.A. Thompson %A W. Liu, %A E. Chun %A V. Katritch %A H. Wu %A E. Vardy %A X.-P. Huang %A C. Trapella %A R. Guerrini %A G. Calo %A B.L. Roth %A V. Cherezov %A R.C. Stevens %J Nature %V 485 %P 395–399 %O doi:10.1038/nature11085 %D 2012 %T Structure of the δ-opioid receptor bound to naltrindole %A S. Granier %A A. Manglik %A A.C. Kruse %A T.S. Kobilka %A F.S. Thian %A W.I. Weis %A B.K. Kobilka %J Nature %V 485 %P 400–404 %O doi:10.1038/nature11111 %D 2012