Sequence-Structure alignment of the 10 structurally characterised distinct GPCRs
Here is a joy formatted alignment of the (now) 10 sequence distinct rhodopsin-like GPCR structures - I've selected a representative for those for which there are multiple structures known - usually those that are most complete in terms of lack of disordered loops, etc. The alignment is quite unstable in parts, and several regions are open to interpretation.....
The structures are:
The structures are:
- 2rh1 - human beta-2 adrenergic receptor
- 2vt4 - turkey beta-1 adrenergic receptor
- 3pbl - human dopamine D3 receptor
- 3uon - human muscarinic M2 receptor
- 3rze - human histamine H1 receptor
- 3eml - human adenosine A2a receptor
- 3v2w - human sphingosine-1-phosphate receptor
- 3odu - human CCR4 receptor
- 2i35 - bovine rhodopsin
- 2z73 - squid rhodopsin
10 20 30 40 50 2rh1 ( 29 ) devwvvgmgi 2vt4A ( 40 ) weagmsl 3pblA ( 32 ) yal 3uon ( 20 ) tfevvfivl 3rze ( 28 ) mplvv 3eml ( 3 ) imgssvyit 3v2w ( 17 ) sdyvnydIIvrHYnyTgklnisa ltsv 3oduA ( 27 ) pçfre-------------------------enanfnkiflpt 2i35 ( 1 ) mnGtegpnfyVPfsnktgvVrsPfeapQyyLaepwqFsmlAa 2z73A ( 9 ) etwwyNpsIvVhpHWref--------------dqvpdavYyslGi aaaaa 60 70 80 90 100 2rh1 ( 39 ) vmslivlaIvfgNvlVitAIakferLqtvtnyFItsLAcADlvMGlaVVp 2vt4A ( 47 ) lmalVvllIvagNvlViaAigstqrLqtltnlFItsLAcADlvvGllVVp 3pblA ( 35 ) sYcalilaIvfgNglVcmAVlkeraLqtttnyLVvsLAvADllvAtlVMp 3uon ( 29 ) vagslSlvTiigNilVmvSIkvnrhLqtvnnyflfSLAcADliiGvfSMn 3rze ( 33 ) vlsticlvTvglNllVlyAvrserkLhtvGnlYIvsLSvADliVGavVMp 3eml ( 12 ) vElaiavlAilgNvlVcwAvwlnsnLqnvtnyfVvsLAaADiavGvlAIp 3v2w ( 51 ) vfiliCcfIileNifvlltiwktkkFhrpMYyFIgnLAlSDllaGvaYta 3oduA ( 44 ) iYsiIfltGivgNglvilvMgyqkklrsmtdkYRlhLSvADllFVitLpf 2i35 ( 43 ) yMfllimlGfpiNflTlyVTvqHkkLrtpLNyILlNLAvADlfMVfg-GF 2z73A ( 40 ) fIgiCgiiGcggNgiViyLFtktksLqtpanmFiinLAfSDftFSlvNGf aaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaaaa aaa 110 120 130 140 150 2rh1 ( 89 ) fgaahilm-kmWtfgnfwçefWTSiDVlCVTASIeTLcvIAvdryfAIts 2vt4A ( 97 ) fgatlvvr-gtWlwgsflçelWTSlDVlCVTAsIeTLcvIAiDrylaits 3pblA ( 85 ) wvvylevtggvWnfsricÇdvFVTlDVmMcTAsIwNLCaISidRytAVvm 3uon ( 79 ) lytlytvi-gyWplgpvvÇdlWlalDYvVSNAsVmNLliiSfdryfcvtk 3rze ( 83 ) mnilyllm-skwsLgrplÇlfWLSmDYVASTASIfSVfiLCiDryrsvqq 3eml ( 62 ) faitist---gfçaaçhgÇLfiACfvLVLtQSsIfsLlaIAiDryiairi 3v2w ( 101 ) Nlllsga--tTykLtPaqWFlREGsMFvALSASVfSLlaIAieryitmlk 3oduA ( 94 ) WavDAva---nWyfgnflÇkaVHviYTVNlYSSVwILAfISlDRylAiVh 2i35 ( 92 ) ttTlyTSlhGyFvfgptgÇnlEGffATlGGEIALWSLVvLaiERyvvvCk 2z73A ( 90 ) plMtiSCflkkWifgfaaÇkvYGfiGGiFGFMsIMTMAMiSiDrynViGr aaaaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 160 170 180 190 200 2rh1 ( 138 ) pfkyqSl---ltknkArviilmvwivSgltSflpIqmhwyr-----athq 2vt4A ( 146 ) pfryqsl---mtrarAkviictvwaiSalvSflpImmhwWr-----dedp 3pblA ( 135 ) pvhyqhgtgqsscrrValmitavwvlAfaVSc-pLlfgfNtTg------- 3uon ( 128 ) pltypvk---rttkmAgmmiaaAwvlSfilwapaIlfwqfivg------- 3rze ( 132 ) plrylky---rtktrAsatilgawflSfl-WvipIlgwnh 3eml ( 109 ) plryngl---vtgtrAkgiiaicwvlSfaiGltPmlgwNnÇgq 3v2w ( 156 ) nnfrlfllisacwviSlilGglPimgwn----------- 3oduA ( 141 ) atn----sqrprkllAekvVyvgVwipAlllT-ipDfif-Anvsead--- 2i35 ( 142 ) pmsn----frfgenhAimgvafTwvmAlaCAapPlvgwSrYIPE------ 2z73A ( 140 ) pmaas---kkMshrrAfimiifVwlwSvlwAigPifgwGaYtLE------ aaaaaaaaaaaaaaaaaaa aaa 210 220 230 240 250 2rh1 ( 180 ) eAinÇyae-etçÇdff--------TnqayaiasSivSFyvplviMvfvYs 2vt4A ( 188 ) qAlkçyqd-pgçÇdfv--------TnrayaiasSiiSFyipLliMifval 3pblA ( 177 ) --------dptvÇsIs---------npdFViySSvvSFylPfgvTvlvya 3uon ( 168 ) ----vrtVedgeÇyIqff------snaavtfgtAiaaFylpviiMtvlyw 3rze ( 175 ) rredkÇeTdfy------dvtwfkvmtaiinFylPtllMlwfya 3eml ( 156 ) sqgçgegqvaÇlFedVV-----pmnYMVyfNffacVlvplllMlgvYl 3v2w ( 184 ) ----ÇisalssÇSTVLP-------LYhkhYIlfCTtvFtllllsIvilYc 3oduA ( 182 ) --------dryiÇdrfyp---ndlwvvvfqfqhimvglilPgivIlsCyc 2i35 ( 182 ) -------gMQCSÇGIDYYTpheetnNesFViyMfvvHfiiPlivIffCyg 2z73A ( 181 ) -------GVLCNÇSFdYIsr--dsttrsNIlcMFilGffgPiliiffCyf aaaaaaaaaaa aaaaaaaaaaaa 260 270 280 290 300 2rh1 ( 221 ) rVfqeakrql kfclkeHkaLktlgiIMgtFt 2vt4A ( 229 ) rvyreakeq irehkalktlgiImgvFt 3pblA ( 210 ) rIyvvlkqrrrk-----------------gvplrekkatqMVaiVlgaFi 3uon ( 208 ) hisrasksri pppsrekkvtrtilaIllaFi 3rze ( 212 ) kIykaVrqhc lhmnrerkaakQLgfIMaaFi 3eml ( 199 ) rIflaarrql rstlqkevhaAkSlaiIvglFa 3v2w ( 223 ) riyslvrtr asrssenvaLlkTViiVLsvFi 3oduA ( 221 ) iIisklshs kghqkrkalktTviLilaFf 2i35 ( 225 ) qlvfTvkeaAaq------------qqesattqkaekevtrMViiMviaFl 2z73A ( 222 ) nIvmsvsnhekemaamakrlnakelrkaqaganaemrlAkIsivIVsqFl aaaaaa aaaaaaaaaaaaaaaaa 310 320 330 340 350 2rh1 ( 284 ) lcWlpFFiVNivhviqdn----------lirkevyillNwiGYvNSgfNp 2vt4A ( 301 ) lCWlpFFlvnivnvfnrd----------lvpdwlfvafnwlGYAnSAmnp 3pblA ( 340 ) vCWlpFFltHvlnthçqt--------ç-hvspelysattwlGYvNsalNP 3uon ( 398 ) itWapYNvmVlintfçap--------ç--ipntvwtiGywlCYinstiNp 3rze ( 426 ) lCWipYFiffmviafçkn--------ç--cnehlhmftiWlGYiNStlNP 3eml ( 244 ) lcwlpLHiiNcftffçpd--------çshaplwlMylAivlSHtnSvvnP 3v2w ( 267 ) acwapLFiLLllDvgçkvk------tç--diLfrAeyfLvlAvlNSgtNP 3oduA ( 250 ) acWlpyyigisidsfilleiikqgçefentvhkwisitEAlAFfHCclNp 2i35 ( 263 ) iCWlpYAgvAfyIfthqgsd---------fgPifMTipAFfAKtSAvyNP 2z73A ( 272 ) lSWspYAvvAllAQfgplew---------VtpyaAQlpVMfAKaSaihNP aaaaaaaaaaaaaaa aaaaaaaaaaaaa aaa 360 370 380 390 400 2rh1 ( 324 ) liYc-rspdfriAfqellcl 2vt4A ( 341 ) iiYc-rspdfrkAfkrlla 3pblA ( 381 ) viYttfnieFrkAflkilsc 3uon ( 438 ) acYalcnatFkktfkhllm 3rze ( 466 ) liYplCnenFkktfkrilhi 3eml ( 286 ) fiYayrireFrqTfrkIirshvlrq 3v2w ( 309 ) iiytltNkemrrafiri 3oduA ( 300 ) ilyaflgakfktsaqhalts 2i35 ( 304 ) viYimmnkqFrnCmvtTlccgkn dde 2z73A ( 313 ) miYsvsHpkFreAIsqtfpwvLtccqfddketeddkdaeteipage aaaaaa aaaaaaaaaa