Sequence-Structure alignment of the 11 structurally characterised distinct GPCRs
Here is a joy formatted alignment of the (now) 11 sequence distinct rhodopsin-like GPCR structures - I've selected a representative for those for which there are multiple structures known - usually those that are most complete in terms of lack of disordered loops, etc. The alignment is quite unstable in parts, and several regions are open to interpretation.....
The structures are:
The structures are:
- 3uon - human muscarinic M2 receptor
- 4daj - rat muscarinic M3 receptor
- 3rze - human histamine H1 receptor
- 2rh1 - human beta-2 adrenergic receptor
- 2vt4 - turkey beta-1 adrenergic receptor
- 3pbl - human dopamine D3 receptor
- 2ydv - human adenosine A2a receptor
- 3v2w - human sphingosine-1-phosphate receptor
- 3odu - human CCR4 receptor
- 2i35 - bovine rhodopsin
- 2z73 - squid rhodopsin
The next to be released structures will almost certainly be the mu-opioid receptor (PDB code 4DKL) and the kappa-opioid receptor (PDB code 4DJH), which are on hold awaiting publication.
10 20 30 40 50
3uon ( 20 ) tfevvfivl
4dajA ( 64 ) iwqvvfiaf
3rze ( 28 ) mplvv
2rh1 ( 29 ) devwvvgmgi
2vt4A ( 40 ) weagmsl
3pblA ( 32 ) yal
2ydv ( 3 ) imgssvYit
3v2w ( 17 ) sdyvnydIIvrHYnyTgklnisa ltsv
3oduA ( 27 ) pçfre-------------------------enanfnkiflpt
1u19A ( 1 ) mnGtegpnfyVPfsnktgvVrsPFeapQyyLaepwqFsmlAa
2z73A ( 9 ) etwwyNpsIvVhpHWref--------------dqvpdavYyslGi
aaaaa
60 70 80 90 100
3uon ( 29 ) vagslSlvTiigNilVmvSIkvnrhLqtvnnyflfSLAcADliiGvfSMn
4dajA ( 73 ) ltgflAlvTiigNilVivAFkvnkqLktvnnyFllSLAcADliIGviSMn
3rze ( 33 ) vlsticlvTvglNllVlyAvrserkLhtvGnlYIvsLSvADliVGavVMp
2rh1 ( 39 ) vmslivlaIvfgNvlVitAIakferLqtvtnyFItsLAcADlvMGlaVVp
2vt4A ( 47 ) lmalVvllIvagNvlViaAigstqrLqtltnlFItsLAcADlvvGllVVp
3pblA ( 35 ) sYcalilaIvfgNglVcmAVlkeraLqtttnyLVvsLAvADllvAtlVMp
2ydv ( 12 ) vElaiavlAilgNvlVcwAvwlnsnLqnvtnyFVvsAAaADilVGvlAIp
3v2w ( 51 ) vfiliCcfIileNifvlltiwktkkFhrpMYyFIgnLAlSDllaGvaYta
3oduA ( 44 ) iYsiIfltGivgNglvilvMgyqkklrsmtdkYRlhLSvADllFVitLpf
1u19A ( 43 ) yMflLimlGfpiNflTlyVTvqHkkLrtplNyILlnLAvADlfMVfg-GF
2z73A ( 40 ) fIgiCgiiGcggNgiViyLFtktksLqtpanmFiinLAfSDftFSlvNGf
aaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaaaa aaa
110 120 130 140 150
3uon ( 79 ) lytlytvi-gyWplgpvvÇdlWlalDYvVSNAsVmNLliiSfdryfcvtk
4dajA ( 123 ) lFttyiim-nrWalgnlaÇdlwLSiDYvASNAsVmNLlvISfDryfsitr
3rze ( 83 ) mnilyllm-skwsLgrplÇlfWLSmDYVASTASIfSVfiLCiDryrsvqq
2rh1 ( 89 ) fgaahilm-kmWtfgnfwçefWTSiDVlCVTASIeTLcvIAvdryfAIts
2vt4A ( 97 ) fgatlvvr-gtWlwgsflçelWTSlDVlCVTAsIeTLcvIAiDrylaits
3pblA ( 85 ) wvvylevtggvWnfsricÇdvFVTlDVmMcTAsIwNLCaISidRytAVvm
2ydv ( 62 ) faiaIst---GfçaaçhgÇLfiACfVLVLTASSIfSLlaIAiDryiairi
3v2w ( 101 ) Nlllsga--tTykLtPaqWFlREGsMFvALSASVfSLlaIAieryitmlk
3oduA ( 94 ) WavDAva---nWyfgnflÇkaVHviYTVNlYSSVwILAfISlDRylAiVh
1u19A ( 92 ) tTTlyTSlhGyFvfgptGÇnlEGffATLGGEIaLWSLvvLaieRyvvVck
2z73A ( 90 ) plMtiSCflkkWifgfaaÇkvYGfiGGiFGFMsIMTMAMiSiDrynViGr
aaaaaaa aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
160 170 180 190 200
3uon ( 128 ) pltypvk---rttkmAgmmiaaAwvlSfilwapaIlfwqfivg-------
4dajA ( 172 ) pltyrak---rttkrAgvmiglAwviSfvlWApaIlfwqyfvg-------
3rze ( 132 ) plrylky---rtktrAsatilgawflSfl-WvipIlgwnh
2rh1 ( 138 ) pfkyqSl---ltknkArviilmvwivSgltSflpIqmhwyr-----athq
2vt4A ( 146 ) pfryqsl---mtrarAkviictvwaiSalvSflpImmhwWr-----dedp
3pblA ( 135 ) pvhyqhgtgqsscrrValmitavwvlAfaVSc-pLlfgfNtTg-------
2ydv ( 109 ) plryngl---vtgtrAkgiiaicwvlSfaIGltPmlgwnnÇgqp--kegk
3v2w ( 156 ) nnfrlfllisacwviSlilGglPimgwn-----------
3oduA ( 141 ) atn----sqrprkllAekvVyvgVwipAlllT-ipDfif-Anvsead---
1u19A ( 142 ) pmsn----frfgenhaimgvafTwvmAlaCAapPlvgwSrYIPE------
2z73A ( 140 ) pmaas---kkMshrrAfimiifVwlwSvlwAigPifgwGaYtLE------
aaaaaaaaaaaaaaaaaaa
210 220 230 240 250
3uon ( 168 ) ----vrtVedgeÇyIqff------snaavtfgtAiaaFylpviiMtvlyw
4dajA ( 212 ) ----krtVppgeÇfIqfl------septitfgtAiaaFymPvtiMtilyw
3rze ( 175 ) rredkÇeTdfy------dvtwfkvmtaiinFylPtllMlwfya
2rh1 ( 180 ) eAinÇyae-etçÇdff--------TnqayaiasSivSFyvplviMvfvYs
2vt4A ( 188 ) qAlkçyqd-pgçÇdfv--------TnrayaiasSiiSFyipLliMifval
3pblA ( 177 ) --------dptvÇsIs---------npdFViySSvvSFylPfgvTvlvya
2ydv ( 154 ) ahsqgÇgegqvAÇlFedVV-----pmnYMVyfNffaCVlvPlllMlgvyl
3v2w ( 184 ) ----ÇisalssÇSTVLP-------LYhkhYIlfCTtvFtllllsIvilYc
3oduA ( 182 ) --------dryiÇdrfyp---ndlwvvvfqfqhimvglilPgivIlsCyc
1u19A ( 182 ) -------GMQCSÇGIDYYTpheetnNesFViyMfvvHfiiPlivIffcyg
2z73A ( 181 ) -------GVLCNÇSFdYIsr--dsttrsNIlcMFilGffgPiliiffCyf
aaaaaaaaaaa aaaaaaaaaaaa
260 270 280 290 300
3uon ( 208 ) hisrasksri pppsrekkvtrtilaIllaFi
4dajA ( 252 ) rIyketek like aqTlsaIllaFi
3rze ( 212 ) kIykaVrqhc lhmnrerkaakQLgfIMaaFi
2rh1 ( 221 ) rVfqeakrql kfclkeHkaLktlgiIMgtFt
2vt4A ( 229 ) rvyreakeq irehkalktlgiImgvFt
3pblA ( 210 ) rIyvvlkqrrrk-----------------gvplrekkatqMVaiVlgaFi
2ydv ( 199 ) rIflaarrqlkqmesq stlqkevhaakSLaiIvglFa
3v2w ( 223 ) riyslvrtr asrssenvaLlkTViiVLsvFi
3oduA ( 221 ) iIisklshs kghqkrkalktTviLilaFf
1u19A ( 225 ) qLvftvkeaaaq------------qqesattqkaekevTrMviiMviaFl
2z73A ( 222 ) nIvmsvsnhekemaamakrlnakelrkaqaganaemrlAkIsivIVsqFl
aaaaa aaaaaaaaaaaaaaaaa
310 320 330 340 350
3uon ( 398 ) itWapYNvmVlintfçap--------ç--ipntvwtiGywlCYinstiNp
4dajA ( 501 ) itWtpyNimVlvntfçds--------ç--ipktywnlgywlCYiNStvNP
3rze ( 426 ) lCWipYFiffmviafçkn--------ç--cnehlhmftiWlGYiNStlNP
2rh1 ( 284 ) lcWlpFFiVNivhviqdn----------lirkevyillNwiGYvNSgfNp
2vt4A ( 301 ) lCWlpFFlvnivnvfnrd----------lvpdwlfvafnwlGYAnSAmnp
3pblA ( 340 ) vCWlpFFltHvlnthçqt--------ç-hvspelysattwlGYvNsalNP
2ydv ( 244 ) lCWlpLHiiNcftffçpd--------çshaplwlMylAivlSHtNSvvNP
3v2w ( 267 ) acwapLFiLLllDvgçkvk------tç--diLfrAeyfLvlAvlNSgtNP
3oduA ( 250 ) acWlpyyigisidsfilleiikqgçefentvhkwisitEAlAFfHCclNp
1u19A ( 263 ) iCWlpYAgvAfyIfthqgsd---------fgpifMTipAFfAKtSAvyNP
2z73A ( 272 ) lSWspYAvvAllAQfgplew---------VtpyaAQlpVMfAKaSaihNP
aaaaaaaaaaaaaaa aaaaaaaaaaaaa aaa
360 370 380 390 400
3uon ( 438 ) acYalcnatFkktfkhllm
4dajA ( 541 ) vcYalcnktFrttfkt
3rze ( 466 ) liYplCnenFkktfkrilhi
2rh1 ( 324 ) liYc-rspdfriAfqellcl
2vt4A ( 341 ) iiYc-rspdfrkAfkrlla
3pblA ( 381 ) viYttfnieFrkAflkilsc
2ydv ( 286 ) fiyAyrireFrqTFrkiirshvlrqqepfkaa
3v2w ( 309 ) iiytltNkemrrafiri
3oduA ( 300 ) ilyaflgakfktsaqhalts
1u19A ( 304 ) viYimmnkqFrnCmvttlccgknplgddeasttVsktetsqvapa
2z73A ( 313 ) miYsvsHpkFreAIsqtfpwvLtccqfddketeddkdaeteipage
aaaaa aaaaaaaaaa